The current standard method, histopathology, is imperfect since it only involves looking at a very small sample of the regional lymph nodes under a microscope. There is no way to know whether occult metastases are present in the rest of the tissue.
Analysis of the lymph nodes for GUCY2C expression using a technique called reverse transcriptase-polymerase chain reaction (RT-PCR), amplifies the sensitivity to detect cancer cells compared to histopathology.
Read more in http://www.sciencedaily.com/releases/2009/02/090217173036.htm.
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